ProtoCOL 3 - Automatic colony counting and zone measuring

ProtoCOL 3

ProtoCOL 3 is the next generation instrument for colony counting, zone measurements, (inhibition and AST), membranes and a range of other applications.

Categories: , Industries: Environment & Water, Food & Drink, Pharmaceutical, Veterinary
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AST Chromogenic ID Colony counting Spiral Plates Zone Reading 3 Year warranty Camera CFR part11 CLSI Eucast ISO 9001:2008 LIMS Operating System TIFF USB XLS

Overview

ProtoCOL 3 is the next generation instrument for colony counting, zone measurements (inhibition and antibiotic susceptibility testing), membranes, PETRIFILM™ plates and a range of other applications including: spiral plates, OPKA (opsonophagocytic killing assay), SBA (serum bactericidal assay), Multi-sector, Multi-well, SRD (single radial immunodiffusion) and Ames. The new design now enables plates of up to 150mm diameter to be automatically read at the press of a button. Designed for use across a wide range of applications, ProtoCOL 3 will count colonies as small as 43 microns (0.043mm) or measure zones accurately to 0.5mm with a theoretical detection limit of 0.1mm.

ProtoCOL 3 has a unique LED lighting system configured for exceptional illumination of all sample types. High definition, colour images taken with a 1.4 mega pixel scientific grade CCD camera ensure that even the smallest colony can be seen and counted, while zone measurements are fast, accurate and reproducible.

ProtoCOL 3 is available with a mounted touch screen processor which enables ease of control while the easy-to-use software produces highly accurate results. Alternatively, the ProtoCOL 3 can now be used with a new or existing stand-alone desktop or laptop computer via USB connection – no additional PCI cards are required.

The new ProtoCOL 3 comes inclusive with both colony counting and zone measuring ability. To extend its range of applications, inexpensive additional modules can be added to the software for Spiral, OPKA, SBA, Multi-sector, Multi-well, Ames and antibiotic susceptibility testing (eAST). The Inhibition Zone software module is supplied as standard. Results can be automatically transferred to Excel or a LIMS system. The system can also be used with 1D barcodes. All data generated is GLP/GMP compliant with a full audit trail and can be used to produce professional reports. Each system comes complete with 2 validation plates.

PETRIFILMTM is a trademark of 3M Company. 3M Company is not affiliated with Synbiosis and does not endorse or certify Synbiosis’s products.

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Why Buy?

Why buy this product?

  • High throughput
    ProtoCOL 3 is typically used when large numbers of plates need to be counted or measured rapidly and accurately. The ProtoCOL 3 is therefore perfect for high throughput applications. All counting and measuring functions are automatic with results instantly sent to the internal reporting system.
  • Colour imaging
    The unique LED lighting in the system gives full colour images. The colour classification feature of the ProtoCOL 3 software allows for an infinite range of colour options on the same plate. A choice of sample holders is included for both light and dark backgrounds, depending on sample type.
  • Wide range of applications
    The system can be configured for a wide range of plate types. These include pour plates, spiral plates, multi-well, SBA, OPKA, Ames, inhibition zones, AST and SRD. Plates up to 150mm can be used on the standard unit, while an optional external scanning device can be added should larger plates need to be processed.
  • Extensive report generation
    One of the major benefits of an automated system is that reports can be generated within the device. ProtoCOL 3 will produce reports that can easily be exported to Excel at the touch of a button.
  • Traceability
    All the data generated has a full audit trail. All report generation is traceable and has an audit ‘lock’. Data is CFR compliant and the system can be connected to a LIMS if required.
  • Sensitivity
    ProtoCOL 3 uses a highly sensitive CCD camera with a high resolution. Colonies as small as 43 microns (0.043mm) can be easily counted and zones can be accurately measured to 0.5mm with a theoretical detection limit of 0.1mm.

Specifications

ProtoCOL 3 ProtoCOL 3 Plus
Construction Ergonomic housing constructed in high density foam

Integral CCD camera and lens

Ergonomic housing constructed in high density foam

Integral CCD camera and lens

Mounted all-in-one PC

Light shield 2 sliding doors to prevent excessive ambient light 2 sliding doors to prevent excessive ambient light
Camera 1.4m pixel scientific grade CCD camera

USB integral camera with f1.2 lens

1.4m pixel scientific grade CCD camera

USB integral camera with f1.2 lens

Resolution For standard 150mm Petri dish, smallest detectable colony is 43 microns For standard 150mm Petri dish, smallest detectable colony is 43 microns
Imaging 3 channel capture for colour images 3 channel camera for colour images
Lighting Unique 3 channel (red, green, blue) LED lighting

Multi-array LED lighting (computer controlled)

Lower lighting with upper reflective lighting for all applications

Unique 3 channel (red, green, blue) LED lighting

Multi-array LED lighting (computer controlled)

Lower lighting with upper reflective lighting for all applications

External connections All-in-one touch panel PC
Windows 7 Professional 32 bit edition service pack 1
Intel Core i3-350M CPU @ 2.26GHz
4GB DDR3 RAM
160GB Hard drive
Intel HD Graphics
17″ SXGA panel screen with native optimal resolution of 1280 x 1024
4 x hi-speed USB 2.0 ports
Measurement modes Colony counting and zone measurement Colony counting and zone measurement
Count modes Separation of touching colonies, exclusion areas, colour mode, shape mode, size mode Separation of touching colonies, exclusion areas, colour mode, shape mode, size mode
Software Win7/10 compatible Win7/10 compatible
Database SQL database stores all data and image locations SQL database stores all data and image locations
Dimensions 450mm (h) x 500mm (d) x 400mm (w) 700mm (h) x 500mm (d) x 400mm (w)
Weight 20kg 30kg

Software

Software

  • The ProtoCOL 3 software has an extensive array of features and can cover a wide range of applications. Colony counting and zone measurement are standard on the ProtoCOL 3
  • ProtoCOL 3 software is fully WIN7 compatible and is available in a range of different languages
  • All functions are accessed from a single window for each step
  • Automatic imaging functions ensure perfect images without the need for any optimisation of contrast and sensitivity
  • Automatic separation of touching colonies is included
  • Advanced grid elimination function is included for filters and PETRIFILMTM  plates
  • Colonies can be marked with choice of shapes, including cross, circle, square or triangle, for clear and precise visualisation
  • An advanced polygonal exclusion tool is available to eliminate specific areas
  • The system can be ‘trained’ to exclude writing on plates, debris, bubbles or other unwanted features
  • Full editing functions for the adding or deleting of colonies and the manual measurement of zones are included
  • Colonies as small as 0.043mm can be detected while zones can be measured accurately to 0.5mm with a theoretical detection limit of 0.1mm.
  • A built-in database enables the sharing of results across individuals, laboratories and even facilities
  • All results are automatically archived and can be printed
  • All results are fully traceable and have an audit trail
  • Compliant with the requirements of 21 CFR Part 11

PETRIFILM TM is a trademark of 3M Company. 3M Company is not affiliated with Synbiosis and does not endorse or certify Synbiosis’s products.

Applications

Wide range of applications

The system can be configured for a wide range of plate types. These include:

  • Pour plates
  • Spiral plates
  • Multi-well
  • SBA (serum bactericidal assay)
  • OPKA (opsonophagocytic killing assay)
  • Ames
  • Inhibition zones
  • AST (antibiotic susceptibility testing)
  • SRD (single radial immunodiffusion)

 

  • For customers with GFP applications, Syngene (our sister company) has the complete solution – please look at our range of advanced gel doc systems by visiting the website

Choosing & purchasing

Choose your model

ProtoCOL 3 Plus (with all-in-one PC)

ProtoCOL 3 Plus is a fully configured system with a top mounted ‘all-in-one’ touch screen PC. The all-in-one PC has a integral hard drive. Spare USB ports for use with memory sticks or other USB driven devices like bar code readers and scanners are easily accessible on the side of the unit.

The system includes ProtoCOL 3 software for colony counting, zone measurement (inhibition zones and AST), membranes and PETRIFILMTM plates

Additional software modules that can be added are:

Spiral plates
OPKA
SBA
Multi-sector
Multi-well
SRD
AMES
Statistical analysis

ProtoCOL 3 (for use with stand-alone PC)

ProtoCOL 3 is also available as a unit without a processor. This allows the user to simply connect the system to a new or existing PC. ProtoCOL 3 software is complete with set-up and calibration installation instructions. It can be installed onto the PC which is connected to the ProtoCOL via USB.

The system includes ProtoCOL 3 software for colony counting, zone measurements (inhibition zones and AST), membranes and PETRIFILMTM plates

Additional software modules that can be added are:

Spiral plates
OPKA
SBA
Multi-sector
Multi-well
SRD
AMES
Statistical analysis

With your own PC you can decide on the processor and monitor you want and, of course, you can upgrade or add additional hardware of your choice whenever you wish. With your own PC you can also choose your operating system including WIN7.

Stand-alone software

We are now able to supply stand-alone ProtoCOL 3 software which can be loaded onto any PC. A flat bed scanner can be used to capture images. This is a low cost way of getting many of the functions of a ProtoCOL 3 into your laboratory.

Purchasing

ProtoCOL 3 Plus

ProtoCOL 3 for plates up to 150mm, supplied with two plate holders and validation kit
Includes all-in-one PC
Software configured for colony counting, zone measurement, membranes and PETRIFILMTM plates
WIN7 compatible

ProtoCOL 3

ProtoCOL 3 for plates up to 150mm, supplied with two plate holders and validation kit
USB connection to stand-alone computer
ProtoCOL 3 software for colony counting, zone measurement, membranes and PETRIFILMTM plates
WIN7 compatible

Optional application modules:-

Spiral plates
OPKA
SBA
Multi-sector
Multi-well
SRD
Ames
Statistical analysis

PETRIFILM TM is a trademark of 3M Company. 3M Company is not affiliated with Synbiosis and does not endorse or certify Synbiosis’s products.

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