Protocol for multiplexed opsonophagocytic killing assay (UAB-MOPA) for antibodies against Streptococcus pneumoniae

Opsonization assay has become an important tool in assessing pneumococcal vaccine immunogenicity (reference 1). To facilitate the widespread use of the opsonization assay, we have prepared a detailed protocol for 4-fold multiplexed opsonophagocytic killing assay (UAB-MOPA). Although this protocol describes the multiplexed assay, this protocol can be used as a single serotype assay with the minor changes described in Note 9 in Assay Notes section. Also, this protocol can be used with minor alterations (e.g., a different serial dilution factor or different number of wells per sample). Additional information on this assay can be found in publications (references 2 and 3) and at a web site (www.vaccine.uab.edu).