SRID – Single Radial Immunodiffusion

/SRID – Single Radial Immunodiffusion

SRID – Single Radial Immunodiffusion

2018-06-26T11:47:14+00:00March 28th, 2018|

Radial Immunodiffusion is a technique used extensively for the quantitative estimation of antigens.

Antibody of known specificity is distributed evenly in an agar gel and a sample containing the antigen of interest is placed in a well within the gel. Antigen then diffuses radially from the well and a precipitin ring forms at the point of antibody-antigen equilibrium. Antigen concentrations are determined by measuring the diameter of precipitin rings and extrapolating using standard curves.

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