Technical literature

Technical papers archive

Tuesday 3rd May 2005

Simplified method to automatically count bacterial colony forming unit (Opsonophagocytosis assay, Bacterial colony counting)

Bacterial colony counting is a significant technical hurdle for vaccine studies as well as various microbiological studies. We now show that an automated colony counter can process images obtained with a digital camera or document scanner and that any laboratory can efficiently have bacterial colonies enumerated by sending the images to a laboratory with a colony counter via internet. (This Research Paper appeared in the Journal of Immunological Methods 302 (2005) 99 - 102).

Wednesday 2nd March 2005

Modulation of gene expression by promoter mutants of the λ cI857/pRM/pR system

The following paper cites the use of ProtoCOL colony counter.

Monday 1st November 2004

Automating color colony counting

To check the safety of foods, microbiologists routinely liquefy and plate samples onto agar plates. They then examine the food for potentially pathogenic bacteria such as Salmonella, E.coli 0157, Bacillus cereus, and Listeria monocytogenes, as well as anaerobes such as Campylobacter. On the basis of the level of potentially harmful bacteria in the sample, decisions are made as to whether to market the product.

Wednesday 1st September 2004

Automated colony counting proves accurate

Producing new anti-microbial therapies and vaccines to treat biological terrorism threats such as anthrax and smallpox has become a priority. Since colony counts provide the data on which the efficacy of this type of treatment is based, it is essential to obtain accurate counts in the shortest possible time.

Sunday 1st August 2004

Colonies are clearly visible - new assay allows automated colony counting

Evaluations of pneumococcal vaccines using the OPKA (opsonophagocytic-killing assay) require the determination of antibody responses, which is commonly performed by enumerating surviving bacterial colonies.

Monday 1st December 2003

A novel method for automating zone measurement of SRD assays

Single radial immunodiffusion (SRD) is a simple yet powerful technique that is routinely used in many clinical laboratories for a wide variety of analyses. Despite its simplicity, the technique as currently practiced suffers from a major drawback due to the fact that in general, the measurement of the reaction zones generated by the assay is performed manually. This is a task that is time-consuming and error-prone. To overcome these problems, a novel method of automating inhibition zone measurement has been developed. The new system has been extensively tested and compared with the standard, manual method. We report here the performance of the new method in the assay of the potency of influenza vaccines.

Monday 1st September 2003

Microbiological quality control procedures improved through use of modern image analysis

One of the main functions of microbiology laboratories in modern pharmaceutical companies is to carry out routine quality control testing. This involves testing various types of samples for the presence of contaminating bacteria. Although the initial screening tests are performed using relatively simple culture plates, the fact that large numbers of such plates have to be screened means that accurate archiving and transmission of the results can be a challenge. Modern image handling and archiving systems can dramatically improve the reliability of microbiology QC systems and ensure speedy transmission of potentially vital data to the appropriate site. The case study presented in this article describes how AstraZeneca have successfully incorporated the use of image handling systems into their microbiology QC procedures.

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