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Colony counting - ProtoCOL
1) A comparison of manual and automated colony counting
2) Streamlining the selection of recombinant antibodies
3) How rapid quality testing could mean the difference between selling milk or throwing it away before it even reaches the consumer
4) Mobile skin microbiology laboratory for clinical trials and research offers accurate results and alleviates resource shortages
5) Detecting the source of a food poisoning outbreak
6) Identification of a Dithiazoline Inhibitor of Escherichia coli L,D-Carboxypeptidase A
7) Host Genetics of Bordetella pertussis Infection in Mice: Significance of Toll-Like Receptor 4 in Genetic Susceptibility and Pathobiology
8) Evaluation of a Select Strain of Lactobacillus delbrueckii subsp. lactis as a Biological Control Agent for Pathogens on Fresh-Cut Vegetables stored at 7 degrees C
9) Daptomycin Dose-Effect Relationship against Resistant Gram-Positive Organisms
10) Examination of the potential genotoxicity of pure capsaicin in bacterial mutation, chromosome aberration, and rodent micronucleus tests
Colour colony counting - ProtoCOL
11) Automating color colony counting
Colony counting - aCOLyte
12) New generation of colony counters remove operator errors in the lab
13) Automated colony counting proves accurate
14) Microbiological quality control procedures improved through use of modern image analysis
15) Streamlining the selection of recombinant antibodies
SRD assays - ProtoCOL
16) A novel method for automating zone measurement of SRD assays
17) Automed zone measurement in single radial immunodiffusion assays promises rapid quality control of influenza vaccines
OPKA assay - ProtoCOL
18) Colonies are clearly visible - evaluations of pneumococcal vaccines using the OPKA (opsonophagocytic-killing assay) require the determination of antibody responses, which is commonly performed by enumerating surviving bacterial colonies
19) Simplified method to automatically count bacterial colony forming unit (Opsonophagocytosis assay OPKA, Bacterial colony counting)
20) Quadruple Opsonophagocytic Killing Assay OPKA (MOPA4)
21) Efficiency of a Pneumococcal Opsonophagocytic Killing Assay (OPKA) improved by multiplexing and by coloring colonies
A comparison of manual and automated colony counting
Counting four different types of serially diluted bacterial colonies on 75 pour plates compared automated and manual counting systems. The results were analysed statistically and showed that the automated counting method produced results that were not significantly different from the manually counting method at the 95% confidence level. (The following article appeared in the September 2006 issue of Drug Plus International - www.drugplusinternational.com)
Automating color colony counting
To check the safety of foods, microbiologists routinely liquefy and plate samples onto agar plates. They then examine the food for potentially pathogenic bacteria such as Salmonella, E.coli 0157, Bacillus cereus, and Listeria monocytogenes, as well as anaerobes such as Campylobacter. On the basis of the level of potentially harmful bacteria in the sample, decisions are made as to whether to market the product.
New generation of colony counters remove operator errors in the lab
Laboratories with a high throughput of samples now demand faster results while maintaining accuracy.
Automated colony counting proves accurate
Producing new anti-microbial therapies and vaccines to treat biological terrorism threats such as anthrax and smallpox has become a priority. Since colony counts provide the data on which the efficacy of this type of treatment is based, it is essential to obtain accurate counts in the shortest possible time.
Microbiological quality control procedures improved through use of modern image analysis
One of the main functions of microbiology laboratories in modern pharmaceutical companies is to carry out routine quality control testing. This involves testing various types of samples for the presence of contaminating bacteria. Although the initial screening tests are performed using relatively simple culture plates, the fact that large numbers of such plates have to be screened means that accurate archiving and transmission of the results can be a challenge. Modern image handling and archiving systems can dramatically improve the reliability of microbiology QC systems and ensure speedy transmission of potentially vital data to the appropriate site. The case study presented in this article describes how AstraZeneca have successfully incorporated the use of image handling systems into their microbiology QC procedures.
A novel method for automating zone measurement of SRD assays
Single radial immunodiffusion (SRD) is a simple yet powerful technique that is routinely used in many clinical laboratories for a wide variety of analyses. Despite its simplicity, the technique as currently practiced suffers from a major drawback due to the fact that in general, the measurement of the reaction zones generated by the assay is performed manually. This is a task that is time-consuming and error-prone. To overcome these problems, a novel method of automating inhibition zone measurement has been developed. The new system has been extensively tested and compared with the standard, manual method. We report here the performance of the new method in the assay of the potency of influenza vaccines.
Streamlining the selection of recombinant antibodies
Antibodies make good drug candidates because their natural role in the body’s immune system means that they are potent, specific, well tolerated and can be readily raised to a variety of different targets.
How rapid quality testing could mean the difference between selling milk or throwing it away before it even reaches the consumer
According to the US Department of Agriculture billions of pounds of milk are destroyed every year - why is that? Well one reason is the milk’s past its sell by date and another is it’s infected by potentially harmful microbes. As a supervisor in a busy contract dairy testing lab, my primary concern is to get it checked out rapidly so manufacturers have the maximum chance of shifting milk off the shelf and onto your breakfast cereal.
Colonies are clearly visible - evaluations of pneumococcal vaccines using the OPKA (opsonophagocytic-killing assay) require the determination of antibody responses, which is commonly performed by enumerating surviving bacterial colonies
New assay allows automated colony counting.
Simplified method to automatically count bacterial colony forming unit (Opsonophagocytosis assay, Bacterial colony counting)
Bacterial colony counting is a significant technical hurdle for vaccine studies as well as various microbiological studies. We now show that an automated colony counter can process images obtained with a digital camera or document scanner and that any laboratory can efficiently have bacterial colonies enumerated by sending the images to a laboratory with a colony counter via internet. (This Research Paper appeared in the Journal of Immunological Methods 302 (2005) 99 - 102).
Mobile skin microbiology laboratory for clinical trials and research offers accurate results and alleviates resource shortages
Abnormalities in the cutaneous microbial population of propionibacteria, coagulase-negative staphylococci and the yeast, Malassezia furfur are either implicated or directly associated with dermatological disorders such as acne, eczema, erysipelas, impetigo and folliculitis.
Automed zone measurement in single radial immunodiffusion assays promises rapid quality control of influenza vaccines
In the UK, people over 65 and those considered to be in high-risk groups are routinely offered an inoculation to protect them against the flu (influenza) virus. If the concentration of the inactivated viral vaccine is incorrect then those inoculated may be insufficiently protected, which could prove fatal among some of these vulnerable groups.
Detecting the source of a food poisoning outbreak
With just three full-time staff and around 3,000 food and water samples to process each year, the UKAS accredited Microbiology Department at the Western General Hospital, Edinburgh, needs to utilise the most efficient and effective methods available to meet increasing demands. Food samples tested include raw and cooked meats from local butchers and food manufacturers plus processed foods sent in by the environmental health department. These are commonly examined for aerobic bacteria such as Salmonella, E.coli 0157, Bacillus cereus and Listeria monocytogenes as well as anaerobes, for example, Campylobacter.
Identification of a Dithiazoline Inhibitor of Escherichia coli L,D-Carboxypeptidase A
There is a link to ProtoCOL in the following article.
Quadruple Opsonophagocytic Killing Assay (MOPA4)
There is a link to ProtoCOL in the following article.
Host Genetics of Bordetella pertussis Infection in Mice: Significance of Toll-Like Receptor 4 in Genetic Susceptibility and Pathobiology
There is a link to ProtoCOL in the following article.
Evaluation of a Select Strain of Lactobacillus delbrueckii subsp. lactis as a Biological Control Agent for Pathogens on Fresh-Cut Vegetables Stored at 7 degrees C
There is a link to ProtoCOL in the following article.
Examination of the potential genotoxicity of pure capsaicin in bacterial mutation, chromosome aberration, and rodent micronucleus tests
There is a link to ProtoCOL in the following article.
Daptomycin Dose-Effect Relationship against Resistant Gram-Positive Organisms
There is a link to ProtoCOL in the following article.
Efficiency of a Pneumococcal Opsonophagocytic Killing Assay (OPKA) improved by multiplexing and by coloring colonies
There is a link to ProtoCOL in the following article.
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