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Manual methods of enumerating the OPKA assay are not only time consuming but can lead to reading and transcription errors, especially when counting such large numbers of S. pneumoniae colonies, many of which can be less than 1 mm in diameter.
To overcome the difficulties associated with manually counting colonies on large OPKA assay plates, Synbiosis provides an additional stand and platform containing a high resolution camera which can be integrated with a ProtoCOL 2 system. The system can evaluate the colonies on large OPKA plates because its software automatically compensates for different agar thickness and artefacts such as bubbles or debris that can occur during overlaying and can even enumerate touching colonies and colonies of different sizes.
The ProtoCOL produces results from OPKA assays which are compliant with GCP (Good Clinical Practice) guidelines so can be presented to regulatory authorities such as the European Medicines Control Agency (EMEA) and Food and Drug Administration (FDA).
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